| NMKL newsletter |
|
|
| Nr. 51/02, October 2002 | ||
NMKL
is arranging
New NMKL methods:
No.
173, 2002: ASH, gravimetric determination in foods
No.
174, 2002: SHIGELLA SPP. PCR method for detection in foods.
The 56th Annual Meeting of NMKL on Åland 24th - 27th of August 2002
No.
173, 2002: ASH, gravimetric determination in foods.
This
method was elaborated and collaboratively tested by Cand. pharm. Arne Højgård
Jensen, Danish Veterinary and Food Administration, Regional Veterinary and Food
Control Authority, Århus, Denmark.
14
nordic laboratories participated in the testing, which included 7 foods (green
beans, semi-skimmed milk, fromage frais, tuna, rye bread, wheat bran and salami)
with ash contents of between 0.45 - 5.25 g/100 g. Thus, this method is suitable
for ash contents higher than 0.45 g /100 g.
The
method is intended for the determination of ash content in foods for use when
calculating their energy content.
The
principle of this method is that the sample is ashed to constant weight in a
muffle kiln at 550 °C. If necessary, samples are dried prior to ashing.
No
174, 2002: SHIGELLA SPP. PCR method for detection in foods.
Cand.
pharm. Christian Fermér and Associated Professor Roland Lindqvist in the
National Food Administration of Sweden drew up this method.
Christian Fermér organized the collaborative method performance study.
The
method is suitable for the detection of Shigella bacteria in food, and
has been validated for the detection of Sh. flexneri and Sh. sonnei
in prawns, iceberg lettuce and blue cheese. The method has been validated twice
with 11 and 9 participating laboratories respectively.
The
method detects Shigella bacteria both with and without the virulence plasmid.
The presence of Shigella bacteria is qualitatively determined, and the
method has a sensitivity of 0.2 - 40 cfu per gram of sample (5 - 1000 cfu per 25
grams of sample).
The
sample is analysed with PCR after preparation. The used primers target a gene,
ipaH, located both on the bacterial chromosome and on the virulence plasmid.
This sequence is considered specific for Shigella bacteria and enteroinvasive E.
coli (EIEC).
![]()
NMKL
would like to thank Arne Højgård Jensen, Christian Fermér and Roland
Lindqvist and their institutes, for their considerable efforts in elaborating
and arranging the collaborative studies. NMKL
would also like to thank all the participating laboratories, as well as the NMKL
contacts for their valuable comments and input.
Institutes
arranging proficiency testing schemes on foods open to all, are hereby
encouraged to inform the general secretariat of NMKL about their web page. This
will enable us to create links from NMKL’s home page to these activities,
which are of great interest to all laboratories.
Would
you like to subscribe to the NMKL Newsletter?
An
NMKL subscription is still only NOK 500, and includes all the methods published
by NMKL annually, i. e. 6 methods
or more.
The subscription does not include procedures and reports.
How about a new chronological index for the method binders?
Tip:
Open the NMKL home page and under Publications, find and print the chronological
publication index.
NMKL
are considering offering courses on the following topics in 2003:
Control
of microbiological substrates
Sampling
- "educating" inspectors
In the Autumn of 2003 (week 41) NMKL will, in co-operation with The Royal Veterinary and Agricultural University in Denmark, host the following seminar:
New
Instrumental Analytical Methods and Data Analysis in Food Quality Control
The seminar will be held at The Royal Veterinary and Agricultural
University, Denmark.
The
56th Annual Meeting of NMKL on Åland 24th - 27th of
August 2002
The
Finnish National Committee of NMKL invited NMKL members to hold their 56th
Annual Meeting in Mariehamn on the island of Åland. The annual meeting gathers
the NMKL members, consisting of 60 selected experts within analysis of food from
Denmark, Finland, Iceland, Norway and Sweden. The members represent food control
authorities, the food industry and different research institutes dealing with
food analyses. Every country has its own national committee with a chairperson,
secretary and members. All members put a lot of work into developing methods and
contributing to their collaborative validation. A lot of the work is done after
ordinary working hours, and with great commitment and enthusiasm. This can also
be seen through the annual meetings, which are held in conjunction with
weekends. The picture below shows the chairpersons of the 5 NMKL national
committees.

From
the left (rear): Associate Professor Tone Normann Asp (Norwegian chairperson),
Managing Director Ole Bjørn Jensen (Danish chairman and chairman of NMKL),
Laboratory manager Franklín Georgsson (Icelandic chairman).
From the left (front): Special planner Harriet Wallin (Finnish chairperson and
also hosting the 56th annual meeting), Laboratory manager Ulla Edberg (Swedish
chairperson and acting chairperson of NMKL during parts of the meeting)
The
meeting covers all of the topics in the NMKL working programme, currently
counting 57 topics, 41 methodical topics and 16 projects within microbiological,
chemical and sensorial topics. A
list of all the topics and their locations in the NMKL system - the NMKL working
programme - is available on the NMKL home page: www.nmkl.org.
Most of the work at the annual meetings is performed in the relevant
committees, lead by the chairpersons/acting chairperson of the sub committees,
Dr. Maija Hatakka, Senior Scientist Marit Rødbotten and Professor Kåre
Julshamn.
![]() |
![]() |
![]() |
|
Chairperson
of Sub Committee 2:
Microbiological topics: |
Chairman
of Sub Committee 3: Chemical
topics: Professor Kåre Julshamn, Institute of Nutrition, Directorate of
Fisheries , Norway |
Acting
Chairperson of Sub Committee
4: Sensorial topics: Senior Scientist Marit Rødbotten, Matforsk, Norway |
Administrative
matters are treated in Sub Committee 1, lead by chairman of NMKL, Ole Bjørn
Jensen. The chairman of NMKL could not attend the whole of this year’s annual
meeting. In his absence, Ulla Edberg acted as chairperson.
The annual meetings are also a very important arena for discussing relevant analytical problems and for network building.
Information
from NMKL’s own officials, as well as from invited representatives of NMKL’s
main cooperating partners, is another important element of the annual meeting.
The following work groups in EK-Livs (The Nordic Committee of Senior Officials
for Food Issues) were represented: NNK (Nordic work group for food control), NKE
(Nordic work group for food and nutritional issues), NNM (Nordic work group for
food microbiology and risk assessment) and NNT (Nordic food group for toxicology
and risk assessment). Furthermore, reports were presented from the work of the
European standardisation organisation within food research (CEN/TC 275), in
which NMKL is involved in all of the work groups. This year too, representatives
from AOAC International/AOAC Research Insitute and IDF (International Dairy
Federation) spoke of their organisations’ activities, and took an active part
in the work performed during the meeting.

Invited
guests (from the left): Scott
Coates, AOAC International/AOAC RI and Gudmund Bråthen, IDF.
Available
NMKL procedures:
No
|
Year
|
Title |
Language
|
|
1 |
1995 |
Performance check and in-house calibration of
analytical balances. |
English,
Swedish |
|
2 |
1995 |
Performance check and in-house calibration of
thermometers. |
English,
Swedish |
|
3 |
1996 |
Control charts and control materials in
internal quality control in food chemical laboratories. |
English,
Swedish |
|
4 |
1996 |
Validation of chemical analytical methods. |
English,
Norwegian, Spanish |
|
5 |
1997 |
Estimation and expression of measurement
uncertainty in chemical analysis. |
English,
Swedish, Spanish |
|
6 |
1998 |
Guide on quality assurance in sensorial
laboratories |
Danish,
Finnish |
|
7 |
1998 |
Checking of UV/VIS spectrophotometers |
Danish,
English |
|
8 |
2002, 2nd Ed. |
Measurement of uncertainty in microbiological
examination of foods. |
Norwegian
(soon in English) |
|
9 |
2001 |
Evaluation of results derived from the
analysis of certified reference materials. |
English, Swedish |
|
10 |
2001 |
Control of Microbiological Media |
English, Norwegian |
|
11 |
2002 |
Sensorial evaluation of drinking water |
Norwegian (will be translated to English) |
|
12 |
2002 |
Guide on sampling for analysis of food |
English, Norwegian, (soon Finnish and Polish) |