NMKL methods

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Biological methods

 Title:

No.

Year/edition

Botulinum toxin. Determination in foods, blood and other test materials. 

79

1991, 2nd Ed.

Paralytic shellfish poison. Biological determination in the common mussel and other bivalves.

81

1986, 2nd Ed.

 

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Chemical and physical methods

 Title:

No.

Year/edition

Acid value/free fatty acids. Determination in fats.

38

2001, 4rd Ed. 

Acidity, titratable. Determination in milk and cream (Routine method).

107

1984 

Alpha-amylase activity. Determination in cereals and cereal products

77

1970 

Arsenic. Determination in foods and food additives by molybdenum blue method.

112

1985 

Arsenic.  Determination in seafood by electrothermal atomic absorption spectrometry (ET AAS) after microwave digestion.

166

2000

Ash. Determination in grain and flour.

7

1987, 2nd Ed. 

Ash. Determination in milk and milk products.

108

1984 

Ash. Gravimetric determination in foods.

128

1989 

Ash. Gravimetric determination in foods.

173

2002

Aspartame and diketopiperazin. Determination by HPLC.

142

1992 

Benzoic acid and sorbic acid in foods. Quantitative determination by gas chromatography.

103

1984

Benzoic acid and sorbic acid in foods. Amendments: Safety precaution.

103

1991 

Benzoic acid. Determination in foods.

2

1979, 3rd Ed. 

Benzoic acid, sorbic acid and p-hydroxybenzoic acid esters. Liquid chromatographic determination in foods.

124

1997, 2nd Ed. 

Carmine. Liquid chromatographic determination in foods.

157

1997 

Chloride. Determination in meat and fish products.

89

1974

Chloride. Determination in meat and fish products. Corrections.

89

1986 

Cholecalciferol  (vitamin D3) and Ergocalciferol (vitamin D2). Determination by HPLC in foodstuffs.

167

2000

Colouring matters, oil-soluble, synthetic. Isolation and identification.

32

1959 

Colouring matters, water-soluble, synthetic. Isolation and identification.

31

1959 

Colouring matters, water-soluble, synthetic. Isolation and identification.

114

1985 

Colours, synthetic, water-soluble. Liquid chromatographic determination in foods.

130

1989 

Colours, synthetic, water-soluble, semi-quantitative. Determination by chromatography and spectrophotometry .

134

1990 

Cyclamate. Spectrophotometric determination in foods.

123

1998, 2. Ed. 

DRY MATTER in foodstuffs. The vacuum method.

169

2002

Ergosterol.  Determination by HPLC in cereals

172

2002

Fat. Determination according to SBR in meat and meat products.

131

1989 

Fat. Determination in foods.

160

1998 

Fat. Determination of content in milk by the Gerber method.

40

1961 

Formaldehyde. Determination in foods.

54

1964 

Fructose, glucose and saccharose. Liquid chromatographic determination in fruit and vegetable products.

148

1993

Gravimetric determination of fat in milk, cream and milk powder

10

2001, 3rd Ed.

Glycoalkaloids. Liquid chromatographic determination in potatoes.

159

1997 

Histamine. Determination in fish.

99

1981 

Histamine. Qualitative thin-layer chromatographic screening method for fish.

118

1988, 2nd Ed. 

HYDROXYPROLINE. Colorimetric determination as a measure of collagen in meat and meat products.

127

2002, 2nd Ed. 

Inefficient cleansing. Chemical method for detection on cups, dishes and plates

4

1962, 2nd Ed. 

Iodine value. Determination in fats (Wij's method).

39

1972, 2nd Ed. 

Iron. Photometric determination in food.

116

1985 

L-Glutamic acid and monosodium glutamate. Enzymatic determination in meat and fish and in soups.

138

1991 

Lactos and galactose. Enzymatic determination in foods.

155

1996 

Lead and cadmium. Release from ceramic articles.

94

1991, 2nd Ed. 

Magnesium and calcium. Determination by atomic absorption spectrometry after wet digestion in a microwave oven.

153

1996 

MERCURY. Determination in Seafood by Flow Injection Cold Vapour Atomic Absorption Spectrometry (FI-CVAAS) after Microwave Digestion.

170

2002
Metals.  Determination by atomic absorption spectrophotometry after wet digestion in a microwave oven.

161

1998

Metals. Determination by atomic absorption spectrophotometry in foodstuffs.

139

1991 

Moisture and ash. Gravimetric determination in meat and meat products.

23

1991, 3rd Ed. 

Moisture. Determination in bread.

14

1953 

n-Dodecyl gallate (DG), n-octyl gallate (OG), n-propyl gallate (PG) and nordihydroguaiaretic acid (NDGA). Detection in fat. TLC method.

84

1984, 2nd Ed.  

Net content and drained weight. Determination in preserved fruits and vegetables.

55

1980, 2nd Ed. 

Nitrite and nitrate. Determination in meat products, brines and salt mixtures (Tentative method).

100

1982  

Nitrite and Nitrate.  Nitrite and/or nitrate in foodstuffs by ion chromatography.

165

2000

Nitrite: I Unspecific titrimetric routine method for the determination of nitrite in "nitrite salt" and "nitrite salpetre". II Determination of nitrite in "nitrite salt" and "nitrite salpetre", and in meat, fish and brine.

49

1963 

 

Nitrogen. Determination in foods and feeds according to Kjeldahl.

6

1976, 3rd Ed. 

Ochratoxin A. Liquid chromato-graphic deter-mination in cereal and cereal products.

143

1997, 2nd Ed. 

Oxalic acid. Determination in foods.

63

2000, 2nd Ed. 

Peroxide value. Determination in fat and oil.

158

1997 

Peroxide value. Determination in pure fats, butter and margarine.

45

1962 

Phosphatase activity. Determination for pasteurisation control of milk, cream and whey.

26

1998, 4th, Ed. 

Phosphorus. Spectophotometric determination after dry ashing in food.

57

1994, 2nd Ed. 

Polyphosphates. Detection by paper chromatography in meat products etc.

76

1988, 2nd Ed. 

Saccharin. Liquid chromatographic determination in beverages and sweets .

122

1997, 2nd Ed. 

Salicylic acid in Foods. Qualitative and Quantitative Analysis.

3

1999, 3rd Ed. 

Solids, total. Analytical determination by weight in tomato purée.

28

1958 

Solids, total (water). Gravimetric determination in milk and milk products.

110

1984 

Soluble dry matter. Refractometric determination in tomato purée and tomato paste.

70

1969 

Starch and glucose. Enzymatic determination in foods.

145

1997, 2nd Ed.  

Sugars. Identification by paper chromatography*

33*

1981, 2nd Ed. 

Sulphite. Enzymatic determination in foods.

135

1990 

Sulphite. Spectrophotometric determination in foods.

132

1989 

Sulphurous acid. Determination in foods.

18

1970, 2nd Ed.

Sulphurous acid. Determination in foods. Additions and corrections.

18

1980 

Tin. Determination by atomic absorption in fruits and vegetables.

126

1988 

Tin. Quantitative determination of the total content in food. Spectrophotometric determination with quercetin.

115

1985 

Total dietary fiber. Gravimetric determination after enzymatic degradation in foods (AOAC NMKL method).

129

1989 

Total dietary fibre. Gas chromatographic, colorimetric and gravimetric determination in foods - Uppsala method.

162

1998 

Water activity. Measuring with a polyamide filament hygrometer.

104

1984 

*Method 33: The Method is old and not fitted for routine analysis.  However, the method is very illustrative and therefore very useful for instruction purposes.

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Microbiological methods

 Title:

No.

Year/edition

Aerobic microorganisms and presumptive Enterobacteriaceae.  Enumeration on surfaces and utensils

5

2001, 5th Ed.

Aerobic microorganisms. Determination in foods. 

86

1999, 3rd Ed.

Aerobic microorganisms. Enumeration at 30oC in foods by means of PetrifilmTM plates (AOAC NMKL method). 

146

1993

Aerobic microorganisms (plate count). Determination by the plate count method at 30oC in milk, cream and ice cream. 

27

1994, 3rd Ed.

Aeromonas species, motile. Determination in foods. 

150

1995, 2nd Ed.

Antibacterial substances. Microbiological examination of residues of antibiotics and chemotherapeutics in kidney and muscle from carcasses (4 plate method).

121

2004, 3rd Ed.

Aspergillus flavus and A. parasiticus.  Determination in foods and feeds.

177

2004

Bacillus cereus. Determination in foods. 

67

1997, 4th Ed.

Bacterial count. Determination by direct epifluorescent filter technique (DEFT) in raw minced meat. 

137

2002, 2nd Ed.

Bacteriological examination of fresh and frozen seafood.

96

1994, 2nd Ed.

Bacteriological examination of fresh and frozen seafood. Amendments. 

96

1994

Brochothrix thermosphacta. Determination in meat and meat products. 

141

1991

Butter.  Methods for microbiological examinations of butter.

20

1982, 2nd Ed.

Campylobacter jejuni/coli. Detection in foods. 

119

1990, 2nd Ed.

Canned foods. Method for the examination of keeping quality.

47

1994, 2nd Ed.

Clostridia, sulphite-reducing. Determination in foods. 

56

1994, 3rd Ed.

Clostridium botulinum. Detection in foods and other test materials. 

80

1991, 2nd Ed.

Clostridium perfringens. Determination in foods. 

95

1997, 3rd Ed.

Coliform bacteria and Escherichia coli in foods. Determination by the plate count method with PetrifilmTM plates (AOAC NMKL method). 

147

1993

Coliform bacteria. Detection in foods and fodder. 

44

2001, 5th Ed.

Coliform bacteria, thermotolerant. Enumeration in foods. 

125

1996, 3rd Ed.

Enterobacteriaceae. Determination in foods and feeds. 

144

2000, 2nd Ed.

Enterococcus. Determination in foods. 

68

1992, 2nd Ed.

Escherichia coli O157. Detection in food and feeding stuffs.

164

1999

Folate, biologically active. Microbiological determination in milk and milk products with Lactobacillus casei. 

111

1985

Fusarium. Determination in foods and feedstuffs. 

154

1996

Halophilic and osmophilic microbes ('pink' and 'dun').  Determination in salted fish products. 

171

2002

Lactic acid bacteria. Determination in meat and meat products. 

140

1991

Listeria monocytogenes.  Detection in foods and feeds.

136

2004, 3rd Ed.

Microbiological examination of fully preserved canned foods.

59

1991, 3rd Ed. 

Microorganisms, psychrotrophic. Determination by the colony count method. 

74

2000, 3rd Ed.

Mould and yeasts. Determination in foods. 

98

1995, 3rd Ed.

Pathogenic Vibrio species. Detection and enumeration in foods. 

156

1997, 2nd Ed.

Pathogenic Yersinia enterocolitica. PCR methods for detection in foods. 

163

1998

Pathogenic Yersinia enterocolitica. PCR methods for detection in foods.  Amendments.

163

2001

Sampling and pre-treatment of foods and animal feedstuffs, for quantitative microbiological examination. 

91

2002, 4th Ed.

Salmonella. Detection in foods. 

71

1999, 5th Ed.

Shigella  bacteria. Detection in foods. 

151

1995

Shigella  spp.  PCR method for detection in foods. 

174

2002, 2nd Ed.

Coagulase positive staphylococci. Enumeration in foods.

66

1999, 3rd Ed.

Toxin-producing Penicillium verrucosum. Determination in foods and feedstuffs. 

152

1995

Water activity.  Instrumental Determination by Novasina Electronic Hygrometer and Aqua-Lab Dew Point Instrument.

168

2001

Yersinia enterocolitica. Detection in foods. 

117

1996, 3rd Ed.

 

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Latest update: 23 mars 2004 .