PSP-toxins. Determination of paralytic shellfish poisoning toxins in shellfish by HPLC/fluorescence. (NMKL 182, 2005)
The method is intended for quantitative determination of
paralytic algae toxins that can be present in shellfish.
They are causing the syndrome called Paralytic Shellfish
Poisoning (PSP). The method can determine the PSP-
toxins STX, NEO, GTX1,4 (together), GTX2,3
(together), dcSTX, dcGTX2,3 (together), C1,2
(together) and C3,4 (together).
The toxins are extracted from the shellfish tissue with
1% acetic acid. The extract is cleaned up on a C18
solid phase cartridge and a solid phase ion exchange cartridge
(weak cation exchange) is used to fractionate the toxins
into 3 groups. The toxins are oxidized into fluorescence
compounds that are separated on a C18
HPLC-column and detected with a fluorescence detector.
PSP-myrkyt. Halvaannuttavien simpukkamyrkkyjen määrittäminen simpukasta HPLC/ fluoresenssi tekniikalla. (NMKL 182, 2005)