Phosphorus. Spectophotometric determination after dry ashing in food. (NMKL 57, 2. Ed. 1994)

Description:

The sample is dry-ashed to remove organic material. The acid soluble inorganic residue is used for a colour reaction based on the formation of a blue complex between phosphate and sodium molybdate in presence of ascorbic acid as a reducing agent. The intensity of the blue colour is measured spectrophotometrically at the wavelength 823 ±1 nm. Fosfori. Spektrofotometrinen määrittäminen elintarvikkeista kuivapolton jäkeen. (NMKL 57, 2. painos, 1994)

Kuvaus

Näyte kuivapoltetaan orgaanisen materiaalin poistamiseksi. Happoliukoiselle epäorgaaniselle jäännökselle tehdään värireaktio. Reaktio perustuu sinisen kompleksin [(MoO2·4MoO3)2·H3PO4] muodostumiseen fosfaattien ja natriummolybdaatin reagoidessa askorbiinihapon toimiessa pelkistävänä reagenssina. Sinisen värin intensiteetti mitataan spektrofotometrisesti aallonpituudella 823±1 nm.
Price
450,00kr
  • Produkt ID: NMKL57
  • Copyright: NMKL
  • Edition: 1994, 2. ed.

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