Shigella spp. PCR method for detection in foods. (NMKL 174, 3rd ed., 2016)
The method described here is suitable for the
detection of Shigella bacteria in food and has been
validated for the detection of Sh. flexneri and Sh. sonnei in prawns, iceberg lettuce and blue cheese. The detection includes strains both with and without
the virulence plasmid. The presence of Shigella spp.
is qualitatively determined and the method has a
sensitivity of 0.2-40 cfu per gram of sample (5-1000 cfu per 25 gram sample)
Shigella spp. PCR method (NMKL 174)