New NordVal International certificates

NordVal certificate No. 017, Campylobacter real-time PCR, has been renewed - see the certificate here.



NordVal certificate No. 018, Hygicult® TPC, has been renewed - see the certificate here.



Certificate No. 047 has been extended and now covers determination of Enterococci in both food and water products intended for human consumption. Extensive studies have been carried out on Compact Dry ETC method on food in accordance with the new ISO 16140-2:2016 standard. And the results obtained for the Compact Dry ETC method on water have been recalculated according to this new ISO standard. You can see the certificate "HyServe Compact Dry ETC Method for the Enumeration of Enterococci in Food and Water intended for Human Consumption" here.



NordVal International has recalculated the data for a range of methods according to the ISO 16140-2:2016, and concludes that the methods provide results equivalent to the corresponding ISO method, thus the following certificates have been renewed:

  • HyServe Compact Dry TC Method for the Enumeration of Total Count (No. 033)
  • HyServe Compact Dry ETB Method for the Enumeration of Enterobacteriaceae (No. 034)
  • HyServe Compact Dry CF Method for the Enumeration of Total Coliforms (No. 035)
  • HyServe Compact Dry EC Method for the Enumeration of Escherichia coli and coliforms (No. 036)



RAPID’ Salmonella method, short protocol RAPID’ Salmonella method, double enrichment protocol (NordVal 032)

The NordVal certificate for RAPID’ Salmonella method from Bio-Rad Laboratories in France has been renewed. RAPID’ Salmonella method is a horizontal method for detection of Salmonella spp. The certificate now also includes dry milk and infant formula. See the certficate here.



Salmonella Velox (NordVal 046)

Salmonella Velox has been extensively validated by an expert laboratory and collaboratively validated by 8 laboratories. NordVal International has approved and certified the method. Salmonella Velox is a rapid test that can be carried out within 5.5 hours. The method includes description of an enrichment step, a DNA extraction step, a qPCR step and how to interpret the results.
See the certificate here.



TRANSIA® PLATE Listeria (NordVal 002)

The certificate for TRANSIA® PLATE Listeria from Biocontrol is renewed. The TRANSIA® PLATE Listeria is an ELISA method, tested for detection of Listeria in foods and environmental samples. See the certificate here.

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Updated method on aerobic or anaerobic microorganisms or bacterial spores. Enumeration on blood agar.

New version of NMKL method 189: "Aerobic or anaerobic microorganisms or bacterial spores. Enumeration on blood agar." is available.

This method describes a procedure for monitoring bacteria and/or their spores in the production process and in control of all kinds of food, feed or water. It is a useful tool for determining the heat treatment needed in a production process or the shelf-life of a product. It can also be used as a supplementary analysis when investigating causes of suspected food or waterborne infections or poisoning. This method does not replace Aerobic Plate Count (NMKL No. 86), but it may be used as a supplement. For example, surface inoculation on blood agar may give valuable information regarding the colony morphology and haemolytic activity of the colonies present in the sample.

To this updated edition of NMKL method No. 189 the following changes have been introduced:

  • The chapter “introduction” has been added
  • The incubation time when determining spores has been changed from 8 days to 3 days with a possibility to prolong the incubation up to 6 days, when necessary
  • References have been updated

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Updated method for determination of metals in foodstuffs

NMKL procedure No. 139, "Metals. Determination by atomic absorption spectrophotometry in foodstuffs" has been updated. The change from the previous version is the inclusion of the matrixes included in the collaborative study and the results thereof. In the previous version, chromium and nickel were included. However, the results of the collaborative study for these elements were not satisfactory and hence it is concluded that the method does not seem to be the best for these metals.


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NRL Workshop

Aim of the workshop: To discuss the organisation of NRL, and the duties and requirements imposed by the current and the new Control Regulation 882 on NRL and food analyses.

To share experiences from NRL - How to fulfill the requirements and perform the tasks in an effective way? As the number of laboratories has decreased, and the scopes of the NRL are about to be widened, cooperation will be increasingly important. How can we facilitate Nordic cooperation and networking in these areas?

Date:  8 November 2016

Place:  Norwegian Veterinary Institute/ Norwegian Food Safety Authority, Oslo

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New method on quality control test - Sensory evaluation of marine oils

NMKL method No. 201 describes a sensory quality test of marine oils as an umbrella term for oils extracted from fish, shellfish or marine mammals that are rich in healthy polyunsaturated fatty acids. The method is general and requires that the users of the method set up their own sensory product specifications for the oils they will test or use reference oil.

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Guide for verification of microbiological methods

Before an analytical method is taken into use, e.g. for routine testing, a laboratory must verify that it is capable of performing the analytical method. This includes evaluation of relevant performance characteristics to ensure that the method is fit for purpose within the laboratory.

This procedure provides guidelines on how

  • a laboratory may verify that it is capable of performing an analytical method which has previously been externally validated
  • a laboratory can evaluate the possibility of analysing additional matrices not covered by the primary validation, or include new matrices in the scope of an already verified method
  • a laboratory should plan a verification and elaborate a verification report

The procedure is applicable to the verification of methods already validated properly in an interlaboratory study, and also to well-recognised standardised methods without validation and specified performance characteristics.

The procedure also describes how measurement uncertainty could be established while verifying the method, as the data obtained in the verification can be used in the evaluation thereof.

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Updated method on detection of Shigella spp. with real-time PCR in food

An updated version of method No. 174, "Shigella spp. and Enteroinvasive Escherichia coli (EIEC). Detection with real-time PCR in food" is available. The method has changed from conventional PCR to real-time PCR for the detection of Shigella spp. The real-time PCR has an internal amplification control (IAC) included in the analysis.

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NordVal is an independent, 3rd party that evaluates the quality characteristics and applications of alternative microbiological methods in the analysis of food, water, feedd and environmental samples

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