Seminar on digitalisation of the laboratory - benefits and pitfalls

NMKL arranges a course on digitalisation of the laboratory in Sigtuna, Sweden, on 29 August 2018. The language is English.

Digitalisation provides opportunities for increased quality assurance and smoother data management. However, the process of digitalisation is complicated and systems that do not match expectations are not uncommon. At the seminar, some marketable systems are presented by company representatives. As development progresses rapidly, visions on near-future systems will also be presented.

In the afternoon, representatives from different laboratories will tell about their experiences with digitalising their own lab - what went well and what could have been better? We will also get some tips on what to consider and how to get started. Which are the lowest hanging fruits?

This seminar addresses you who work with improvement of the laboratory, both from a quality assurance and an efficiency perspective.

To see the programme and register for the seminar (deadline:15 June) click here.

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NordVal International's technical committees

NordVal International receives many applications and, consequently, regularly seeks members to its technical committee. If you are interested in contributing to its work, ref. below-mentioned description of functions, please contact the NMKL secretariat. To see description of functions please click here

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New method for determination of methylmercury in foodstuffs

NMKL method No. 202, "Methylmercury: determination by isotope dilution GC-ICPMS in foodstuffs", describes the quantitative determination of mono-methylmercury (MMHg) (10-5000 µg/kg dry weight) in mainly marine biota samples. The principle of the method is that the sample is spiked with an appropriate amount of Hg isotope-enriched MMHg and extracted. After derivatisation the sample is analysed using GC-ICPMS. The GC separates the different mercury species before MMHg is atomised and ionised in the high temperature of the ICP. The ions are extracted from the plasma, and in a mass spectrometer the ions are separated by their mass/charge ratio.

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UPDATED METHOD ON DETECTION OF SHIGELLA SPP. WITH REAL-TIME PCR IN FOOD

An updated version of method No. 174, "Shigella spp. and Enteroinvasive Escherichia coli (EIEC). Detection with real-time PCR in food" is available. The method has changed from conventional PCR to real-time PCR for the detection of Shigella spp. The real-time PCR has an internal amplification control (IAC) included in the analysis.

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Sensory quality control method for drinking water has been updated

NMKL Method 183 describes the sensory quality control method for drinking water. The Finnish / English version of the method has been updated and now includes also test setup and parameters for the appearance of drinking water. Furthermore, the references have been updated. Update of the Norwegian / English version is expected to be ready early 2018.

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Course in verification of microbiological methods

NMKL is arranging a course on verification of microbiological methods (NMKL Procedure 32 from 2017) at the Veterinary Institute, Oslo, on 5 June 2018. The language is Norwegian (PP presentation in English).

To see the programme click here.

If you wish to sign up for this course or have questions in this regard please contact This email address is being protected from spambots. You need JavaScript enabled to view it. by 5 April 2018.

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New NordVal International certificates

New Nordval Certificate 049 issued to BioRad for RAPID´Staph. RAPID’Staph is based on enumeration on an optimised Baird-Parker formula for enumeration of S. aureus in 24 h at 37° C. Some Staphylococcus coagulase positive strains can give colonies with a small or no halo after 24 hours of incubation. An additional incubation of 24 hours could be necessary to see the halo. RAPID´Staph is applicable for a broad range of foods. The validation is performed according to ISO 16140-2 and NordVal Validation Protocol 1. See the certificate here.

 

NordVal International Certificate 041 "Salmonella detection method by real-time PCR" from Eurofins has been renewed.
The method describes a shortened pre-enrichment in buffered peptone water followed by DNA extraction and subsequent real-time PCR analysis. The Salmonella detection method using DNA extraction by boiling or King Fisher is applicable for raw meat and swabs from cattle and pig carcasses. The Salmonella detection method using DNA extraction by King Fisher is also applicable for poultry faeces sock swabs. See the certificate here.

 

New NordVal Certificate 048 issued for LactoSens® 0.01%. DirectSens® has developed a biosensor for the determination of residual lactose in lactose-free milk products. Using a specific enzyme and an electrochemical measurement, the lactose concentration is detected using the LactoSens® Reader (LR01) with sensors from the LactoSens® Test Kit (LK0225). Further information is given in the certificate. A validation of LactoSens has been performed according to the NordVal Validation Protocol 2. The performance of the LactoSens® 0.01% assay was compared against the reference method ISO 22662 IDF 198 Milk and milk products – Determination of lactose content by HPLC. The results obtained are satisfactory and document that there is no statistical difference in the performances between the methods for lactose in milk (and milk products) for levels at or above 0.01%. LactoSens is validated for cow/bovine milk. See the certificate here.

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Updated procedure on sensory analysis of fish and shellfish

A new version of NMKL procedure No. 21, "Guide for sensory analysis of fish and shellfish", is available; list of reference has been updated. The procedure is available in both English and Finnish.

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Verification of microbiological methods

Before an analytical method is taken into use, e.g. for routine testing, a laboratory must verify that it is capable of performing the analytical method. This includes evaluation of relevant performance characteristics to ensure that the method is fit for purpose within the laboratory.

NMKL procedure No. 32 provides guidelines on how

  • a laboratory may verify that it is capable of performing an analytical method which has previously been externally validated
  • a laboratory can evaluate the possibility of analysing additional matrices not covered by the primary validation, or include new matrices in the scope of an already verified method
  • a laboratory should plan a verification and elaborate a verification report

The procedure is applicable to the verification of methods already validated properly in an interlaboratory study, and also to well-recognised standardised methods without validation and specified performance characteristics.

The procedure also describes how measurement uncertainty could be established while verifying the method, as the data obtained in the verification can be used in the evaluation thereof.

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NordVal is an independent, 3rd party that evaluates the quality characteristics and applications of alternative microbiological methods in the analysis of food, water, feedd and environmental samples

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