Course in verification of microbiological methods

NMKL is arranging a course on verification of microbiological methods (NMKL Procedure 32 from 2017) at the Veterinary Institute, Oslo, on 5 June 2018. The language is Norwegian (PP presentation in English).

To see the programme click here.

If you wish to sign up for this course or have questions in this regard please contact This email address is being protected from spambots. You need JavaScript enabled to view it. by 5 April 2018.

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New NordVal International certificates

New NordVal certificate issued for LactoSens® 0.01%. DirectSens® has developed a biosensor for the determination of residual lactose in lactose-free milk products. Using a specific enzyme and an electrochemical measurement, the lactose concentration is detected using the LactoSens® Reader (LR01) with sensors from the LactoSens® Test Kit (LK0225). Further information is given in the certificate. A validation of LactoSens has been performed according to the NordVal Validation Protocol 2. The performance of the LactoSens® 0.01% assay was compared against the reference method ISO 22662 IDF 198 Milk and milk products – Determination of lactose content by HPLC. The results obtained are satisfactory and document that there is no statistical difference in the performances between the methods for lactose in milk (and milk products) for levels at or above 0.01%. LactoSens is validated for cow/bovine milk. See the certificate here.


NordVal International Certificate 038 "iQ-Check® Salmonella II kit" from Bio-Rad has been renewed. NordVal International has recalculated the data for the method according to ISO 16140-2:2016 and concludes that the method for detection of Salmonella  spp. on a broad range of foods provides results equivalent to the results from the reference method. See the certificate here.


NordVal International Certificate 039 "BAX® System Real-Time PCR Assay for Campylobacter jejuni / coli and lari" from Qualicon has been renewed. NordVal International has recalculated the data for the methods according to ISO 16140-2:2016, and concludes that the method for chicken cloacae swabs provides results equivalent to the results from the reference method. See the certificate here


NordVal International Certificate 020 "RAPID’E.coli 2 Agar" from Bio-Rad has been renewed. New studies have been conducted according to ISO 16140-2:2016 and the results reveal that the method provides results equivalent to the results of the reference method for determination of both E.coli and total coliforms in a broad range of foods. See the certificate here


NordVal International Certificate 001 ” TRANSIA® PLATE Salmonella Gold” af BioControl has been renewed.The studies for the extension and the sensitivity study were carried out in 2016 according to the requirements of ISO 16140-2:2016. TRANSIA® PLATE Salmonella Gold is based on a three-step, sandwich-type ELISA (Enzyme Linked ImmunoSorbent Assay) using a microtitre plate with divisible strips coated with antibodies specific for Salmonella and ready-to use reagents. See the certificate here


NordVal certificate No. 046 for DNA Diagnostics method Salmonella Velox has been extended to also cover ready-to-eat og ready-to-reheat products. See the certificate for determination of Salmonella here.



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New method on quality control test - Sensory evaluation of marine oils

NMKL method No. 201 describes a sensory quality test of marine oils as an umbrella term for oils extracted from fish, shellfish or marine mammals that are rich in healthy polyunsaturated fatty acids. The method is general and requires that the users of the method set up their own sensory product specifications for the oils they will test or use reference oil.

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Updated procedure on sensory analysis of fish and shellfish

A new version of NMKL procedure No. 21, "Guide for sensory analysis of fish and shellfish", is available; list of reference has been updated. The procedure is available in both English and Finnish.

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Verification of microbiological methods

Before an analytical method is taken into use, e.g. for routine testing, a laboratory must verify that it is capable of performing the analytical method. This includes evaluation of relevant performance characteristics to ensure that the method is fit for purpose within the laboratory.

NMKL procedure No. 32 provides guidelines on how

  • a laboratory may verify that it is capable of performing an analytical method which has previously been externally validated
  • a laboratory can evaluate the possibility of analysing additional matrices not covered by the primary validation, or include new matrices in the scope of an already verified method
  • a laboratory should plan a verification and elaborate a verification report

The procedure is applicable to the verification of methods already validated properly in an interlaboratory study, and also to well-recognised standardised methods without validation and specified performance characteristics.

The procedure also describes how measurement uncertainty could be established while verifying the method, as the data obtained in the verification can be used in the evaluation thereof.

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NordVal International's technical committees

NordVal International receives many applications and, consequently, regularly seeks members to its technical committee. If you are interested in contributing to its work, ref. below-mentioned description of functions, please contact the NMKL secretariat. To see description of functions please click here

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Updated procedure on quality control of microbiological culture media

A new version of NMKL procedure No. 10, "Quality control of microbiological culture media", is available in English; it has been updated with several clarifications. An updated Scandinavian version is on its way.

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Updated method on aerobic or anaerobic microorganisms or bacterial spores. Enumeration on blood agar.

New version of NMKL method 189: "Aerobic or anaerobic microorganisms or bacterial spores. Enumeration on blood agar." is available.

This method describes a procedure for monitoring bacteria and/or their spores in the production process and in control of all kinds of food, feed or water. It is a useful tool for determining the heat treatment needed in a production process or the shelf-life of a product. It can also be used as a supplementary analysis when investigating causes of suspected food or waterborne infections or poisoning. This method does not replace Aerobic Plate Count (NMKL No. 86), but it may be used as a supplement. For example, surface inoculation on blood agar may give valuable information regarding the colony morphology and haemolytic activity of the colonies present in the sample.

To this updated edition of NMKL method No. 189 the following changes have been introduced:

  • The chapter “introduction” has been added
  • The incubation time when determining spores has been changed from 8 days to 3 days with a possibility to prolong the incubation up to 6 days, when necessary
  • References have been updated

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An updated version of method No. 174, "Shigella spp. and Enteroinvasive Escherichia coli (EIEC). Detection with real-time PCR in food" is available. The method has changed from conventional PCR to real-time PCR for the detection of Shigella spp. The real-time PCR has an internal amplification control (IAC) included in the analysis.

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Sensory quality control method for drinking water has been updated

NMKL Method 183 describes the sensory quality control method for drinking water. The Finnish / English version of the method has been updated and now includes also test setup and parameters for the appearance of drinking water. Furthermore, the references have been updated. Update of the Norwegian / English version is expected to be ready early 2018.

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NordVal is an independent, 3rd party that evaluates the quality characteristics and applications of alternative microbiological methods in the analysis of food, water, feedd and environmental samples

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