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"NMKL 67, 7th Ed. Bacillus cereus group" is withdrawn

17. Dec 2020

Unfortunately, NMKL has decided to withdraw the method “NMKL 67, 7th Ed. 2020 Bacillus cereus group”. We urge customers to use the 6th Ed. until the 7th Ed. has been revised and re-published early 2021.

NMKL acknowledges that a clarification concerning reporting results as ‘Bacillus cereus group’ is needed and that there are discrepancies between the Norwegian/Scandinavian and the English text. If the 7th Ed. has already been applied, please express results as ‘presumptive Bacillus cereus’. There are no known issues with technical performance of the method.

We sincerely apologize for the inconvenience and thank our observant customers for constructive feedback.

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New NordVal International certificates

NordVal logo Dec 2020

Three NordVal International Certificates issued to HyServe for Compact Dry methods have been renewed from December 2020. The Compact Dry methods are ready-to-use chromogenic plates for selective growth and enumeration of the dedicated bacteria and validated for a broad range of foods.

  • NordVal International Certificate 034 for "Compact Dry ETB Method for the Enumeration of Enterobacteriaceae".
  • NordVal International Certificate 035 for "Compact Dry CF Method for the Enumeration of Total Coliforms".
  • NordVal International Certificate 036 for "Compact Dry EC Method for the Enumeration of Escherichia coli and coliforms".

The NordVal International Certificate issued to Bio-Rad for RAPID’Salmonella has been renewed and extended. The method describes detection of Salmonella using a chromogenic agar and confirmation of presumptive colonies, and is validated for a broad range of foods. The extension includes a new protocol to analyse infant formula and infant cereals with or without probiotics including ingredients. An extension study was performed to ensure compliance with ISO 16140-2 and NordVal Validation Protocol 1. NordVal International reviewed the results and concluded that the method provides results equivalent or better than the reference method.

  • NordVal International Certificate 032 "RAPID’Salmonella method, short protocol and RAPID’Salmonella method, double enrichment protocol"

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NMKL general secretariat has moved to Norway

Nov. 2, 2020

As of 1st November 2020, the general secretariat of NMKL has moved from the Technical University of Denmark to the Institute of Marine Research in Norway. We would like to thank the former secretary general Nina Skall Nielsen and the former secretary Kirsten Becker Hansen for their great efforts during these past four years. The new secretary general is Eystein Oveland and the new secretary is Susanna Marie Petrova. We are currently working on updating all contact information on the website, and we thank you for your understanding as it takes some time to get everything in order.

Please note the new e-mail address and phone number:  This email address is being protected from spambots. You need JavaScript enabled to view it. , +47 9026 3022 

Please note that the prices listed as "kr" on the website refer to DKK. We are currently working on clarifying any ambiguities around the currencies in the webshop. Please consult the price list for prices and updated payment terms.

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NMKL procedure No. 19, “Guidelines for sensory evaluation of food packaging”, has been updated

The guidelines in NMKL procedure No. 19 provide several different procedures, which may be applied in the sensory evaluation of food packaging, and point out critical aspects and potential pitfalls of this work. The procedure focuses mainly on the sensory aspects of packaged foods relating to odour and taste evaluation, and consequently does not deal with issues such as the design and practical usability of the packaging. The field of application is wide and covers issues such as continuous production control of incoming materials, evaluation of storage conditions for packaged goods, and controls to ensure compliance with applicable rules and regulations.

The main change in this new version of the procedure is an update of the list of references. In addition, minor editorial corrections are made on the main content.

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New method: NMKL 151 Presumptive Shigella spp. Detection in foods.

NMKL 151 Presumptive Shigella spp. Detection in foods has been reintroduced and updated.

The presence of Shigella in food and the environments is normally of human fecal origin. Shigella is host-specific for higher primates, including humans. Shigella is usually present in foods in low number due to their poor ability to compete. Consequently, they are notoriously difficult to detect in foods. The infectious dose is low, and only 10-100 Shigella are needed to cause infection. Due to the low infectious dose, cross contamination from infected humans to foods and further on to more persons can occur, for example through handling of foods. Person-to-person contamination can also occur. It has been shown that Shigella can survive in weeks and months in or on different foodstuffs.

In this updated method, a step to reduce background flora and ease the isolation of Shigella for some matrices has been introduced. In addition, recommendation regarding which plates to use has been added. This method can be used for subsequent isolation of Shigella spp. after using NMKL 174, 2016, for detection of the virulence factor ipaH in enrichment broth. NMKL 174 can also be used for detection of ipaH in presumptive colonies.

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New payment solution for purchasing publications

Dec 2020

Purchasing publications from NMKL is now easier and more efficient:

  • choose publication(s)
  • go to webshop and complete the order by paying with credit card
  • download the publication directly from the website

Please consult our price list for prices and payment terms.

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NMKL at CCMAS webinar

Nov 2020

NMKL participated in the Codex Committee on Methods of Analysis and Sampling (CCMAS) webinar series November 23-25 and our presentation is now available.

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New NMKL-method: Total folate in food by LC-ESI-MS/MS. Quantification of 6 folate vitamers.

The standardized analytical method for folate in food has traditionally utilized quantification by a microbiological assay, a method that cannot distinguish between the different folate vitamers. This new NMKL method enables quantification of total folate based on quantification of six folate vitamers which contribute to the main folate activity. Besides providing the information about the content of the specific folate vitamers, the method includes fast extraction of folates from food that may be carried out within eight hours. 

The essential part of the method is using a pure γ-glutamyl hydrolase, which enables efficient and fast break-down of the polyglutamates to monoglutamates. LC-ESI-MS/MS quantifies monoglutamates by use of 13C5-labelled internal standards. 

A collaborative study was conducted in summer 2019 with participation of eight laboratories from seven European countries. The method has been successfully validated for total folate determination in strawberries, spinach, lentils, yogurt, liver and infant formula, providing acceptable Horwitz ratio values (0.60-1.94). The results are published in Food Chemistry (Ložnjak Švarc, P; Oveland, E; Strandler, HS; Kariluoto, S; Campos-Giménez, E; Ivarsen, E; Malaviole, I; Motta, C; Rychlik, M; Striegel, L; Jakobsen, J (2020): Collaborative study: Quantification of total folate in food using an efficient single-enzyme extaction combined with LC-MS/MS. Food Chemistry, 333, 127447).

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Updated method: NMKL 183, Quality control test for drinking water

NMKL method 183 describes sensory quality control of drinking water. Water samples are tested against flavour-neutral reference water. If there are deviations, these are registered and a remark is given according to the nomenclature list in the method. The method is updated to now also include test settings and parameters for appearance. In addition, references have been updated.

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3rd edition of procedure on estimation and expression of measurement uncertainty in chemical analysis available in English

When the result of a chemical analysis is presented in an analytical report it is recommended that it should always be reported as a numerical result. For the result to be complete, the measurement uncertainty of the analysis should also be included. In many cases, a result can be very hard to interpret unless accompanied by its uncertainty.

In this updated version of the procedure, guidance on how to handle bias / systematic errors in relation to measurement uncertainty is included. The principles described in Nordtest TR 537 for environmental analyzes have been introduced in the NMKL procedure and examples showing how these calculations are to be performed have been added. Whether one can or should correct for systematic errors is a complex issue, and a new section has been added to explain his. Additionally, minor restructuration and update of references have been undertaken.

A scandinavian version of this 3rd edition will follow.

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NordVal International is an independent third party that evaluates the quality characteristics and applications of alternative chemical and microbiological methods in the analysis of food, water, feed and environmental samples.

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NMKL – NordVal International
c/o Institute of Marine Research
P.O. box 1870 Nordnes
5817 Bergen, Norway

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or by e-mail: post@NMKL.org