Description
This method describes the quantitative, liquid chromatographic (LC) determination of fructose, glucose and saccharose in jam, juice, dried soups, fruit preserves, infant food desserts, beverages etc. The method is intended for samples containing carbohydrates in the following concentration ranges: Fructose: 2 – 30 g/100 g Glucose: 1 – 20 g/100 g Saccharose: 10 – 30 g/100 g Lower concentrations can be detected if the sensitivity of the detector and the pump noise permit. The method is semi-quantitative for lactose and maltose. Note: Samples containing the sugar alcohols sorbitol and mannitol must be analyzed with other methods because these substances interfere with glucose determination. The carbohydrates of the sample are dissolved in water and diluted to suitable concentrations. The final dilution is performed so that the solvent composition corresponds to that of the mobile phase. The carbohydrates are separated isocratically on an amino column and detected with a refractive index detector.