Description
Yersinia enterocolitica is a foodborne bacterial pathogen which may cause yersiniosis in humans worldwide. Y. enterocolitica is widespread in nature and includes a broad spectrum of varieties, of which only a few are pathogenic to humans.
This method describes a horizontal procedure to detect pathogenic Y. enterocolitica (associated with human disease) in food, feed, and environmental samples in the area of food production and food handling. The method has been developed especially to detect the pathogenic bioserotypes of Y. enterocolitica. The method is applicable to products for human consumption, animal feed and environmental samples. The level of detection (LOD) of the method is 9.4 cfu/25 g sample for raw milk, 9.9 cfu/25 g sample for minced meat and 63 cfu/25 g sample for lettuce (see 5.6 for method validation studies and performance characteristics).
Year 2022: The principle of the 4th edition has been completely revised compared to the 3rd edition of the method (cold enrichment). During outbreak investigations, additional procedures may be needed. In such cases, for example, in addition to the general procedure, optional cold enrichment procedure as described in ANNEX 3 may be used.
This NMKL method has been validated in an interlaboratory study.
