Description
The method can be applied to demonstrate the presence of Pink and Dun in salt-cured fish. The lower limit of detection is 100 colony-forming units of the microbe in question per gram of fish. Note that the microbe content may vary greatly on different parts of the fish. Pink and Dun can be demonstrated by inoculating on the appropriate agar media. Samples for Pink are incubated at 37 ºC for 2 – 3 weeks, while samples for dun are incubated at 20 °C (room temperature: 18–24 °C) for 2 weeks or more. Most species will form visible colonies within 4 days. Both Pink and Dun are characterised by their pigmented colonies.